CASP10 (Homo sapiens)
Description [+]
- Synonyms: CASP10, MCH4
- Species: Metazoa;Bilateria;Deuterostoma;Chordata;Vertebrata;Mammalia;Primates;Hominidae; Homo sapiens
- Short gene description: Caspase-10 Precursor (CASP-10)(EC 3.4.22.63)(ICE-like apoptotic protease 4)(Apoptotic protease Mch-4)(FAS-associated death domain protein interleukin-1B-converting enzyme 2)(FLICE2) [Contains Caspase-10 subunit p23/17;Caspase-10 subunit p12] [Source:UniProtKB/Swiss-Prot;Acc:Q92851]
- Family: CASPASE
- Process: apoptosis,
- Pathways: extrinsic pathway,
- Criteria: manually curated
- Curator comment:
- WIKI: CASP10-H_sapiens
References [+]
- In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains.
- Fernandes-Alnemri T, Armstrong RC, Krebs J, Srinivasula SM, Wang L, Bullrich F, Fritz LC, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES
- Emerging evidence suggests that an amplifiable protease cascade consisting of multiple aspartate specific cysteine proteases (ASCPs) is responsible for the apoptotic changes observed in mammalian cells undergoing programmed cell death. Here we describe the cloning of two novel ASCPs from human Jurkat T-lymphocytes. Like other ASCPs, the new proteases, named Mch4 and Mch5, are derived from single chain proenzymes. However, their putative active sites contain a QACQG pentapeptide instead of the QACRG present in ail known ASCPs. Also, their N termini contain FADD-like death effector domains, suggesting possible interaction with FADD. Expression of Mch4 in Escherichia coli produced an active protease that, like other ASCPs, was potently inhibited (Kj = 14 nM) by the tetrapeptide aldehyde DEVD-CHO. Interestingly, both Mch4 and the serine protease granzyme B cleave recombinant proCPP32 and proMch3 at a conserved IXXD-S sequence to produce the large and small subunits of the active proteases. Granzyme B also cleaves proMch4 at a homologous IXXD-A processing sequence to produce mature Mch4. These observations suggest that CPP32 and Mch3 are targets of mature Mch4 protease in apoptotic cells. The presence of the FADD-like domains in Mch4 and Mch5 suggests a role for these proteases in the Fas-apoptotic pathway. In addition, these proteases could participate in the granzyme B apoptotic pathways. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7464-9.
Structure & Sequence [+]
Pfam domains:
(Pfam is a large collection of protein families.)
| Source | Domain Name | Start | End |
|---|---|---|---|
| PFAM A | DED | 20 | 102 |
| PFAM A | DED | 115 | 193 |
| PFAM A | Peptidase_C14 | 284 | 513 |
Protein sequence [+]
CASP10 | Homo sapiens | 9606 | length:522
MKSQGQHWYSSSDKNCKVSFREKLLIIDSNLGVQDVENLKFLCIGLVPNKKLEKSSSASD
VFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERLLPTRQRVSLFRNLLY
ELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQGKIDEDNLTCLEDLCKTVVPK
LLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQTDVKTFLEALPQESWQNKHAGSN
GNRATNGAPSLVSRGMQGASANTLNSETSTKRAAVYRMNRNHRGLCVIVNNHSFTSLKDR
QGTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGR
FGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPE
QAPTSLQDSIPAEADFLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRHEDILSIL
TAVNDDVSRRVDKQGTKKQMPQPAFTLRKKLVFPVPLDALSL
VFEHLLAEDLLSEEDPFFLAELLYIIRQKKLLQHLNCTKEEVERLLPTRQRVSLFRNLLY
ELSEGIDSENLKDMIFLLKDSLPKTEMTSLSFLAFLEKQGKIDEDNLTCLEDLCKTVVPK
LLRNIEKYKREKAIQIVTPPVDKEAESYQGEEELVSQTDVKTFLEALPQESWQNKHAGSN
GNRATNGAPSLVSRGMQGASANTLNSETSTKRAAVYRMNRNHRGLCVIVNNHSFTSLKDR
QGTHKDAEILSHVFQWLGFTVHIHNNVTKVEMEMVLQKQKCNPAHADGDCFVFCILTHGR
FGAVYSSDEALIPIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQPSVSIEADALNPE
QAPTSLQDSIPAEADFLLGLATVPGYVSFRHVEEGSWYIQSLCNHLKKLVPRHEDILSIL
TAVNDDVSRRVDKQGTKKQMPQPAFTLRKKLVFPVPLDALSL
Structure links:
- Smartdomain prediction information: SM00031
- Smartdomain prediction information: SM00115
- Prosite motif and domain information: PS01121
- Prosite motif and domain information: PS01122
- Profile motif and domain profile information: PS50168
- Profile motif and domain profile information: PS50207
- Profile motif and domain profile information: PS50208
- Interpro domain information: Q92851
- PFAM domain and domain family information: Q92851
Evolution [+]
View protein alignment and tree with Jalview:
Explore tree at phylomeDB: Click here.
Homologs list [+]
| Name | Relationship | Species |
|---|---|---|
| CASP10 | orthology | Chicken |
| XR_025516.1 | orthology | Chimpanzee |
| C_intestinalis_ENSCINP00000024485 | orthology | Ciona |
| CASP10 | orthology | Dog |
| T_rubripes_ENSTRUP00000044662 | orthology | Fugu |
| G_aculeatus_ENSGACP00000005791 | orthology | Gasterosteus |
| CASP10 | orthology | Gorilla |
| CASP10 | orthology | Horse |
| CASP10 | orthology | Lyzard |
| CASP10 | orthology | Macaca |
| CASP10 | orthology | Monodelphis |
| Q5RB11_PONPY | orthology | Orangutan |
| CASP10 | orthology | Ornithorhynchus |
| CASP10 | orthology | Rabbit |
| T_nigroviridis_ENSTNIP00000001216 | orthology | Tetraodon |
| ced-3 | orthology | Worm |
| casp10 | orthology | Xenopus |
| CASP10 | orthology | Zebra finch |
| casp8l2 | orthology | Zebrafish |
| A_aegypti_AAEL003444-PA | paralogy | Aedes |
| A_aegypti_AAEL014148-PA | paralogy | Aedes |
| A_aegypti_AAEL014348-PA | paralogy | Aedes |
| CASPS7 | paralogy | Anopheles |
| CASPS6 | paralogy | Anopheles |
| CASPS5 | paralogy | Anopheles |
| CASPS8 | paralogy | Anopheles |
| CASPS4 | paralogy | Anopheles |
| CASPS3 | paralogy | Anopheles |
| Q90WU0_CHICK | paralogy | Chicken |
| NP_001038154.1 | paralogy | Chicken |
| NP_989923.1 | paralogy | Chicken |
| CASP7 | paralogy | Chicken |
| NP_990056.1 | paralogy | Chicken |
| NP_990057.1 | paralogy | Chicken |
| CASP7 | paralogy | Chimpanzee |
| CASP8 | paralogy | Chimpanzee |
| CASP6 | paralogy | Chimpanzee |
| CASP3_PANTR | paralogy | Chimpanzee |
| C_intestinalis_ENSCINP00000003204 | paralogy | Ciona |
| C_intestinalis_ENSCINP00000011493 | paralogy | Ciona |
| C_intestinalis_ENSCINP00000011503 | paralogy | Ciona |
| C_intestinalis_ENSCINP00000025743 | paralogy | Ciona |
| IPI00700977.3 | paralogy | Cow |
| IPI00689801.3 | paralogy | Cow |
| CASP6_BOVIN | paralogy | Cow |
| CASP3_BOVIN | paralogy | Cow |
| NP_001039435.1 | paralogy | Cow |
| IPI00704513.4 | paralogy | Cow |
| CASP7 | paralogy | Dog |
| CASP6 | paralogy | Dog |
| Q38JA9_CANFA | paralogy | Dog |
| Q45T68_CANFA | paralogy | Dog |
| CASP3_CANFA | paralogy | Dog |
| Dcp-1 | paralogy | Fly |
| decay | paralogy | Fly |
| Ice | paralogy | Fly |
| NP_001027871.1 | paralogy | Fugu |
| CASP9 | paralogy | Fugu |
| CASP6 | paralogy | Fugu |
| T_rubripes_ENSTRUP00000024164 | paralogy | Fugu |
| T_rubripes_ENSTRUP00000024467 | paralogy | Fugu |
| CASP7 | paralogy | Fugu |
| CASP9 | paralogy | Gasterosteus |
| CASP7 | paralogy | Gasterosteus |
| CASP2 | paralogy | Gasterosteus |
| G_aculeatus_ENSGACP00000016969 | paralogy | Gasterosteus |
| G_aculeatus_ENSGACP00000016983 | paralogy | Gasterosteus |
| CASP3 (4 of 4) | paralogy | Gasterosteus |
| CASP6 | paralogy | Gasterosteus |
| CASP3 (3 of 4) | paralogy | Gasterosteus |
| CASP3 (2 of 4) | paralogy | Gasterosteus |
| CASP3 (1 of 4) | paralogy | Gasterosteus |
| CASP8 | paralogy | Gorilla |
| CASP7 | paralogy | Gorilla |
| CASP8 | paralogy | Horse |
| CASP6 | paralogy | Horse |
| CASP7 | paralogy | Horse |
| CASP9 | paralogy | Horse |
| Q3S2Z5_HORSE | paralogy | Horse |
| CASP6 | paralogy | Human |
| CASP3 | paralogy | Human |
| CASP8 | paralogy | Human |
| CASP7 | paralogy | Human |
| CASP3 | paralogy | Lyzard |
| CASP7 | paralogy | Lyzard |
| CASP6 | paralogy | Lyzard |
| CASP7 | paralogy | Macaca |
| Q8SPU2_MACMU | paralogy | Macaca |
| CASP8 | paralogy | Macaca |
| CASP9 | paralogy | Macaca |
| Q8JIS9_ORYLA | paralogy | Medaka |
| Q8JIS8_ORYLA | paralogy | Medaka |
| Q56VC9_ORYLA | paralogy | Medaka |
| CASP2 | paralogy | Medaka |
| CASP9 | paralogy | Monodelphis |
| NP_001033059.1 | paralogy | Monodelphis |
| NP_001033061.1 | paralogy | Monodelphis |
| CASP6 | paralogy | Monodelphis |
| CASP8 | paralogy | Monodelphis |
| Casp7 | paralogy | Mouse |
| Casp8 | paralogy | Mouse |
| Casp6 | paralogy | Mouse |
| Casp9 | paralogy | Mouse |
| Casp2 | paralogy | Mouse |
| Casp3 | paralogy | Mouse |
| CASP7 | paralogy | Orangutan |
| Q5RCR7_PONPY | paralogy | Orangutan |
| CASP6 | paralogy | Orangutan |
| CASP3 | paralogy | Orangutan |
| CASP9 | paralogy | Orangutan |
| CASP3 | paralogy | Ornithorhynchus |
| O_anatinus_ENSOANP00000001054 | paralogy | Ornithorhynchus |
| CASP7 | paralogy | Ornithorhynchus |
| CASP6 | paralogy | Ornithorhynchus |
| CASP8 | paralogy | Ornithorhynchus |
| CASP9 | paralogy | Ornithorhynchus |
| CASP8 | paralogy | Rabbit |
| CASP7 | paralogy | Rabbit |
| Casp6 | paralogy | Rat |
| Casp3 | paralogy | Rat |
| Casp8 | paralogy | Rat |
| Casp9 | paralogy | Rat |
| Casp2 | paralogy | Rat |
| Casp7 | paralogy | Rat |
| CASP3 | paralogy | Tetraodon |
| T_nigroviridis_ENSTNIP00000010722 | paralogy | Tetraodon |
| CASP9 | paralogy | Tetraodon |
| CASP6 | paralogy | Tetraodon |
| CASP7 | paralogy | Tetraodon |
| CASP3 | paralogy | Xenopus |
| casp7 | paralogy | Xenopus |
| casp6 | paralogy | Xenopus |
| T_guttata_ENSTGUP00000004278 | paralogy | Zebra finch |
| CASP3 | paralogy | Zebra finch |
| CASP8 | paralogy | Zebra finch |
| T_guttata_ENSTGUP00000011206 | paralogy | Zebra finch |
| CASP9 | paralogy | Zebra finch |
| casp8 | paralogy | Zebrafish |
| casp3a | paralogy | Zebrafish |
| casp7 | paralogy | Zebrafish |
| D_rerio_ENSDARP00000047019 | paralogy | Zebrafish |
| NP_001077331.1 | paralogy | Zebrafish |
| casp3b | paralogy | Zebrafish |
| LOC100000522 | paralogy | Zebrafish |
| A2BGE2_DANRE | paralogy | Zebrafish |
| casp8l1 | paralogy | Zebrafish |
| LOC795066 | paralogy | Zebrafish |
| casp2 | paralogy | Zebrafish |
| casp6l2 | paralogy | Zebrafish |
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Gene Ontology [+]
| GO id | Name | Ontology type | Evidence |
|---|---|---|---|
| GO:0006508 | proteolysis | biological_proccess | IEA |
| GO:0042981 | regulation of apoptosis | biological_proccess | IEA |
| GO:0006915 | apoptosis | biological_proccess | IEA |
| GO:0008624 | induction of apoptosis by extracellular signals | biological_proccess | EXP |
| GO:0004197 | cysteine-type endopeptidase activity | mollecular_function | IEA |
| GO:0005515 | protein binding | mollecular_function | IEA |
| GO:0008234 | cysteine-type peptidase activity | mollecular_function | IEA |
| GO:0042802 | identical protein binding | mollecular_function | IPI |
| GO:0004197 | cysteine-type endopeptidase activity | mollecular_function | TAS |
| GO:0008233 | peptidase activity | mollecular_function | IEA |
Check GO Evidence Codes here
KEGG Pathways [+]
Curated Isoforms [+]
Info from The Vertebrate Genome Annotation (VEGA) database.
(*) Canonical transcript and translation forms.
Information from other databases [+]
- Gene info from HGNC [?] :1500
- Gene related info from GeneCards [?] : CASP10
- Ensembl genome browser [?] : ENSG00000003400
- Expression info from Arrayexpress [?] : ENSG00000003400
- Protein expression from Protein Atlas: [?] ENSG00000003400
- Community gene edition from Wikigenes: [?] 843
Click on [?] for more information.
